Transcriptional Differences between Rhesus Embryonic Stem Cells Generated from In Vitro and In Vivo Derived Embryos
AJ Harvey, S Mao, C Lalancette, SA Krawetz, CA Brenner
PLoS One | Public Library of Science (PLoS) | Published : 2012
Numerous studies have focused on the transcriptional signatures that underlie the maintenance of embryonic stem cell (ESC) pluripotency. However, it remains unclear whether ESC retain transcriptional aberrations seen in in vitro cultured embryos. Here we report the first global transcriptional profile comparison between ESC generated from either in vitro cultured or in vivo derived primate embryos by microarray analysis. Genes involved in pluripotency, oxygen regulation and the cell cycle were downregulated in rhesus ESC generated from in vitro cultured embryos (in vitro ESC). Significantly, several gene differences are similarly downregulated in preimplantation embryos cultured in vitro, wh..View full abstract
Awarded by National Institutes of Health
Awarded by EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT
Awarded by NATIONAL CENTER FOR RESEARCH RESOURCES
This study was supported by the National Institutes of Health grants HD045966, RR015395, RR021881 and HD046553. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.