GPR119 regulates genetic markers of fatty acid oxidation in cultured skeletal muscle myotubes

Abstract

Gene knockout and agonist studies indicate that activation of the G protein-coupled receptor, GPR119, protects against diet-induced obesity and insulin resistance. It is not known if GPR119 activation in skeletal muscle mediates these effects. To address this uncertainty, we measured GPR119 expression in skeletal muscle and determined the effects of PSN632408, a GPR119 agonist, on the expression of genes and proteins required for fatty acid and glucose oxidation in cultured myotubes. GPR119 expression was readily detected in rat skeletal muscle and mRNAs were induced by 12weeks of high-fat feeding. Treatment of cultured mouse C2C12 myotubes with 5μM PSN632408 or 0.5mM palmitate reduced expre..