Journal article

REACTIVITIES OF SEROTYPING MONOCLONAL-ANTIBODIES WITH CULTURE-ADAPTED HUMAN ROTAVIRUSES

RL WARD, MM MCNEAL, JD CLEMENS, DA SACK, M RAO, N HUDA, KY GREEN, AZ KAPIKIAN, BS COULSON, RF BISHOP, HB GREENBERG, G GERNA, GM SCHIFF

Journal of Clinical Microbiology | AMER SOC MICROBIOLOGY | Published : 1991

Abstract

Rotaviruses collected in Bangladesh during 1985 to 1986 were culture adapted and used in a comparative serotyping study with three groups of monoclonal antibodies, all of which reacted with the major neutralization protein (VP7) of serotype 1, 2, 3, or 4. The goals were to determine which monoclonal antibodies most accurately predicted the serotype and why large variations in serotyping efficiencies have occurred with these monoclonal antibodies in previous studies. The 143 rotavirus isolates used in this study belonged to 69 different electropherotypes; and 44, 23, 21, and 55 isolates were identified as serotype 1 through 4, respectively, by neutralization with serotype-specific hyperimmune..

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