Journal article

COMPARISONS OF ROTAVIRUS VP7-TYPING MONOCLONAL-ANTIBODIES BY COMPETITION BINDING ASSAY

P RAJ, DO MATSON, BS COULSON, RF BISHOP, K TANIGUCHI, S URASAWA, HB GREENBERG, MK ESTES

Journal of Clinical Microbiology | AMER SOC MICROBIOLOGY | Published : 1992

Abstract

Three sets of neutralizing monoclonal antibodies (MAbs) used to type the outer capsid protein VP7 of four group A rotavirus serotypes (1 through 4) were compared in competition immunoassays. Reciprocal competition was observed for each of the VP7 type 2-, 3-, and 4-specific MAbs. The VP7 type 1 MAbs exhibited variable competition patterns with other VP7 type 1 MAbs. MAb RV4:3, which has been used to recognize antigenic variants within VP7 type 1 strains, showed reciprocal competition with the four VP7 type 3 MAbs (RV3:1, YO-1E2, 4F8, and 159) using a VP7 type 3 virus (SA11) as antigen. MAb 2C9, also prepared against VP7 type 1, reacted with VP7 type 3 strains and competed with a VP7 type 3 M..

View full abstract

University of Melbourne Researchers