Journal article
Expression profile of genes involved in pathogenesis of pediatric Crohn's disease
WH Sim, J Wagner, DJ Cameron, AG Catto-Smith, RF Bishop, CD Kirkwood
Journal of Gastroenterology and Hepatology Australia | WILEY-BLACKWELL | Published : 2012
Abstract
Background and Aim: Expression profiling of genes specific to pediatric Crohn's Disease (CD) patients was performed to elucidate the molecular mechanisms underlying disease cause and pathogenesis at disease onset. Methods: We used suppressive subtractive hybridization (SSH) and differential screening analysis to profile the mRNA expression patterns of children with CD and age- and sex-matched controls without inflammatory bowel disease (IBD). Results: Sequence analysis of 1000 clones enriched by SSH identified 75 functionally annotated human genes, represented by 430 clones. The 75 genes have potential involvement in gene networks, such as antigen presentation, inflammation, infection mechan..
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Awarded by National Health and Medical Research Council (NHMRC)
Funding Acknowledgements
We would like to thank the children and families for their participation in this study. This project was supported by research grants from the Murdoch Children's Research Institute, The CASS Foundation, The Lynne Quayle Charitable Trust, Equity Trustees Ltd, GlaxoSmithKline Australia, the Victorian Government's Operational Infrastructure Support Program, and by a National Health and Medical Research Council (NHMRC) research grant. Dr Kirkwood is supported by an NHMRC RD Wright Research Fellowship (607347).