Journal article

Inducible system in human hepatoma cell lines for hepatitis C virus production

SP Lim, HM Soo, YH Tan, S Brenner, H Horstmann, JM MacKenzie, ML Ng, SG Lim, WJ Hong

VIROLOGY | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2002

Abstract

We cloned the complete complementary DNA of an isolate of the hepatitis C virus, HCV-S1, into a tetracycline-inducible expression vector and stably transfected it into two human hepatoma cell lines, Huh7 and HepG2. Twenty-six Huh7 and two HepG2-positive clones were obtained after preliminary screening. Two Huh7 (SH-7 and -9) and one HepG2 (G-19) clones were chosen for further characterisation. Expression of HCV proteins in these cells accumulated from 6 h to 4 days posttreatment. Full-length viral plus-strand RNA was detected by Northern analyses. Using RT-PCR and ribonuclease protection assay, we also detected the synthesis of minus-strand HCV RNA. Plus- and minus-strand viral RNA was still..

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University of Melbourne Researchers