Journal article
Epstein-Barr virus genotypes and strains in central nervous system demyelinating disease and Epstein-Barr virus-related illnesses in Australia
MLJ Lay, RM Lucas, C Toi, M Ratnamohan, AL Ponsonby, DE Dwyer
Intervirology | KARGER | Published : 2012
DOI: 10.1159/000334693
Abstract
Objectives: To identify Epstein-Barr virus (EBV) genotypes and strains in samples from individuals with and without a first diagnosis of central nervous system (CNS) demyelinating disease (a possible precursor to multiple sclerosis) and patients with EBV-associated diseases in Australia. Methods: Samples from 55 EBV DNA and serology positive subjects including individuals with (n = 17) and without (n = 21) a first clinical diagnosis of CNS demyelination and patients with EBV-related diseases (n = 17) were examined. EBV genotype and strain were identified by sequence mutations within the Epstein-Barr nuclear antigen-2 region (EBNA-2) using DNA sequence analysis. Results: Both EBV genotypes, A..
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Awarded by National Health & Medical Research Council
Funding Acknowledgements
The authors thank David Gottlieb, Nicole Gilroy, Fiona Kwok and Ian Kay, and the Ausimmune Investigator Group: Caron Chapman, Alan Coulthard, Keith Dear, Terence Dwyer, Trevor J. Kilpatrick, Robyn M. Lucas, Anthony J. McMichael, Michael P. Pender, Anne-Louise Ponsonby, Bruce V. Taylor, Patricia C. Valery, Ingrid A. F. van der Mei and David Williams, for provision of samples. The Ausimmune Study is funded by the National Multiple Sclerosis Society of the USA, the National Health & Medical Research Council (Project Grant 316901) and Multiple Sclerosis Research Australia. We also acknowledge the work of the Ausimmune Study research nurses who undertook sample collection: Susan Agland, Barbara Alexander, Marcia Davis, Zoe Dunlop, Anne Wright, Rosalie Scott, Jannie Selvidge, Marie Steele, Katherine Turner, Brenda Wood and the study project officers, Helen Rodgers and Camilla Jozwick. The author would like to thank Bin Wang for his phylogenetic expertise, Anna Lau and Sharon Chen for manuscript review. We would also like to thank Jacqueline Burrows of Queensland Institute of Medical Research for donating the AG876 strain for our work.