The use of spectroscopic imaging and mapping techniques in the characterisation and study of DLD-1 cell spheroid tumour models
Jenny Z Zhang, Nicole S Bryce, Rainer Siegele, Elizabeth A Carter, David Paterson, Martin D de Jonge, Daryl L Howard, Chris G Ryan, Trevor W Hambley
INTEGRATIVE BIOLOGY | OXFORD UNIV PRESS | Published : 2012
Determining the chemical and biological compositions of the tumour models used in pharmacological studies is crucial for understanding the interactions between the drug molecules and the tumour micro-environment. Conventional techniques for spheroid characterisation require intensive chemical pre-treatments that result in the removal of unbound metabolites. In this study, the spectroscopic techniques, scanning transmission ion microscopy (STIM), proton-induced X-ray emission (PIXE) mapping, scanning X-ray fluorescence microscopy (SXFM), and Fourier transform infrared (FT-IR) imaging were employed to gain complementary information on the compositions of untreated DLD-l cancer cell spheroids. ..View full abstract
Awarded by Australian Research Council
Awarded by Vibrational Spectroscopy Facility
Awarded by Australian Institute of Nuclear Science and Engineering
This work was supported by the Australian Research Council through Discovery grant DP110100461 and was undertaken on the XFM beamline at the Australian Synchrotron, Victoria, Australia; Vibrational Spectroscopy Facility (LIEF LE0883036), the Australian Microscopy & Microanalysis Research Facility and the Histology and Pathology Laboratory at the University of Sydney, N.S.W., Australia. We also acknowledge the Australian Institute of Nuclear Science and Engineering for financial assistance (AWARD no. AINGRA 10110).