The use of DNA from archival dried blood spots with the Infinium HumanMethylation450 array
JiHoon E Joo, Ee Ming Wong, Laura Baglietto, Chol-Hee Jung, Helen Tsimiklis, Daniel J Park, Nicholas C Wong, Dallas R English, John L Hopper, Gianluca Severi, Graham G Giles, Melissa C Southey
BMC BIOTECHNOLOGY | BIOMED CENTRAL LTD | Published : 2013
BACKGROUND: Dried blood (Guthrie card) spots provide an efficient way to collect and store blood specimens. DNA from this source has been utilised for a number of molecular analyses including genome-wide association studies, but only few studies have tested the feasibility of using it for epigenetic applications, particularly at a genome-wide level. RESULTS: In this study, we demonstrate the successful use of DNA isolated from archived dried blood spots for the Infinium HumanMethylation450 Beadchip, along with DNA from matched frozen buffy coats. We obtained high quality and reproducible genome-wide DNA methylation profiles using both sample types. We also report high correlations (r > 0.990..View full abstract
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Awarded by National Health and Medical Research Council of Australia (NHMRC) program grant
The MCCS was supported by VicHealth; Cancer Council Victoria (initial cohort recruitment); and the National Health and Medical Research Council of Australia (NHMRC) program grant 209057, capacity building grant 251533, and enabling grant 396414. MCS is a National Health and Medical Research Council (NHMRC) Senior Research Fellow and a Group Leader of the Victorian Breast Cancer Research Consortium (VBCRC). JLH is funded as an Australia Fellow of the National Health and Medical Research Council (NHMRC) and as a Group Leader of the Victorian Breast Cancer Research Consortium (VBCRC). The authors wish to thank the Australian Genome Research Facility for performing all Infinium HM450 experiments.