Journal article
Myosin heads are displaced from actin filaments in the in situ beating rat heart in early diabetes
MJ Jenkins, JT Pearson, DO Schwenke, AJ Edgley, T Sonobe, Y Fujii, H Ishibashi-Ueda, DJ Kelly, N Yagi, M Shirai
Biophysical Journal | CELL PRESS | Published : 2013
Abstract
Diabetes is independently associated with a specific cardiomyopathy, characterized by impaired cardiac muscle relaxation and force development. Using synchrotron radiation small-angle x-ray scattering, this study investigated in the in situ heart and in real-time whether changes in cross-bridge disposition and myosin interfilament spacing underlie the early development of diabetic cardiomyopathy. Experiments were conducted using anesthetized Sprague-Dawley rats 3 weeks after treatment with either vehicle (control) or streptozotocin (diabetic). Diffraction patterns were recorded during baseline and dobutamine infusions simultaneous with ventricular pressure-volumetry. From these diffraction p..
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Awarded by Australian Nuclear Science and Technology Organisation
Funding Acknowledgements
The authors acknowledge support to M.J.J. and J.T.P from the Access to Major Research Facilities Programme (Australian Nuclear Science and Technology Organisation, AMRFP proposal AS-IA101), to M.S. from the Ministry of Health, Labour and Welfare, and a Grant-in-Aid (A) from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 20590242, 23650213, 23249038), and to D.J.K. from a National Health and Medical Research Council (NH&MRC) Program Grant (No. 546272). The authors declare no conflict of interest.