Journal article
Electron tomography of Plasmodium falciparum merozoites reveals core cellular events that underpin erythrocyte invasion
Eric Hanssen, Chaitali Dekiwadia, David T Riglar, Melanie Rug, Leandro Lemgruber, Alan F Cowman, Marek Cyrklaff, Mikhail Kudryashev, Friedrich Frischknecht, Jake Baum, Stuart A Ralph
CELLULAR MICROBIOLOGY | WILEY | Published : 2013
DOI: 10.1111/cmi.12132
Abstract
Erythrocyte invasion by merozoites forms of the malaria parasite is a key step in the establishment of human malaria disease. To date, efforts to understand cellular events underpinning entry have been limited to insights from non-human parasites, with no studies at sub-micrometer resolution undertaken using the most virulent human malaria parasite, Plasmodium falciparum. This leaves our understanding of the dynamics of merozoite sub-cellular compartments during infectionincomplete, in particular that of the secretory organelles. Using advances in P. falciparum merozoite isolation and new imaging techniques we present a three-dimensional study of invasion using electron microscopy, cryo-elec..
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Grants
Awarded by National Center for Research Resources
Awarded by National Institute of General Medical Sciences from the National Institutes of Health
Awarded by National Health and Medical Research Council of Australia (NHMRC)
Awarded by Australian Research Council
Awarded by Human Frontier Science Organisation (HFSP YI Program)
Awarded by US Department of Energy, Office of Biological and Environmental Research
Awarded by National Center for Research Resources of the National Institutes of Health
Awarded by Australian Research Council (ARC)
Awarded by NATIONAL CENTER FOR RESEARCH RESOURCES
Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
Funding Acknowledgements
The authors thank Carolyn Larabell, and Mark A. Le Gros (University of California, San Francisco) and Christian Knoechel (Lawrence Berkeley National Laboratory) for assistance and advice with X-ray tomography. X-ray tomography was conducted at the National Center for X-ray Tomography, supported by grants from the National Center for Research Resources (5P41RR019664-08) and the National Institute of General Medical Sciences (8 P41 GM103445-08) from the National Institutes of Health. Human erythrocytes were kindly provided by the Red Cross Blood Bank (Melbourne). This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. Funding for the research came from the National Health and Medical Research Council of Australia (NHMRC Project Grants 637340 and APP1047085 JB & SAR), The Australian Research Council (Discovery Project Grant DP120103161), and Human Frontier Science Organisation (HFSP YI Program Grant RGY0071/2011 J.B. and F. F.), the Australian Synchrotron and the Australian Academy of Science, the US Department of Energy, Office of Biological and Environmental Research (DE-AC02-05CH11231), the National Center for Research Resources of the National Institutes of Health (RR019664). Use of the Advanced Light Source was supported by the US Department of Energy, Office of Science. D. T. R. is supported by a Pratt Foundation PhD scholarship through the University of Melbourne; M. R. was supported by the Australian Academy of Science and a grant from the OzeMalaR Network. L. L. was supported by a Postdoctoral fellowship from the University of Heidelberg cluster of excellence CellNetworks. M. K. acknowledges support of the CINA grant from SystemX.ch and from Henning Stahlberg (University of Basel). J.B. and S. A. R. are supported by Australian Research Council (ARC) Future Fellowships (FT100100112 and FT0990350). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors have declared that no conflict of interest exists.