Journal article

Ex-vivo analysis of human Natural Killer T cells demonstrates heterogeneity between tissues and within established CD4( ) and CD4(-) subsets

AC Chan, E Leeansyah, A Cochrane, Y d'Udekem d'Acoz, D Mittag, LC Harrison, DI Godfrey, SP Berzins



Our understanding of human type 1 natural killer T (NKT) cells has been heavily dependent on studies of cells from peripheral blood. These have identified two functionally distinct subsets defined by expression of CD4, although it is widely believed that this underestimates the true number of subsets. Two recent studies supporting this view have provided more detail about diversity of the human NKT cells, but relied on analysis of NKT cells from human blood that had been expanded in vitro prior to analysis. In this study we extend those findings by assessing the heterogeneity of CD4(+) and CD4(-) human NKT cell subsets from peripheral blood, cord blood, thymus and spleen without prior expans..

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Awarded by NHMRC

Funding Acknowledgements

The authors acknowledge the kind donation of tissue for research purposes by donors and their families. This research was supported by an NHMRC Project Grant (no. 454363) and an NHMRC Program Grant (no. 454569). S. P. B. was supported by an NHMRC Career Development Fellowship (no. 454731) and by the Australian Government Collaborative Research Network (CRN). S. P. B. is currently supported as a Dorevitch Senior Research Fellow (at FECRI) and as a Robert H. T. Smith Fellow (Uni of Ballarat). D. I. G. is supported by an NHMRC Senior Principal Research Fellowship (no. 1020770).