Ex-vivo analysis of human Natural Killer T cells demonstrates heterogeneity between tissues and within established CD4( ) and CD4(-) subsets

AC Chan; E Leeansyah; A Cochrane; Y d'Udekem d'Acoz; D Mittag; LC Harrison; DI Godfrey; SP Berzins

Journal article

Ex-vivo analysis of human Natural Killer T cells demonstrates heterogeneity between tissues and within established CD4( ) and CD4(-) subsets

AC Chan, E Leeansyah, A Cochrane, Y d'Udekem d'Acoz, D Mittag, LC Harrison, DI Godfrey, SP Berzins

CLINICAL AND EXPERIMENTAL IMMUNOLOGY | WILEY-BLACKWELL | Published : 2013

DOI: 10.1111/cei.12045

Abstract

Our understanding of human type 1 natural killer T (NKT) cells has been heavily dependent on studies of cells from peripheral blood. These have identified two functionally distinct subsets defined by expression of CD4, although it is widely believed that this underestimates the true number of subsets. Two recent studies supporting this view have provided more detail about diversity of the human NKT cells, but relied on analysis of NKT cells from human blood that had been expanded in vitro prior to analysis. In this study we extend those findings by assessing the heterogeneity of CD4(+) and CD4(-) human NKT cell subsets from peripheral blood, cord blood, thymus and spleen without prior expans..

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University of Melbourne Researchers

Dale Godfrey Author Microbiology and Immunology

Andrew Cochrane Author Paediatrics Royal Children's Hospital

Yves D'Udekem D'Acoz Author Paediatrics Royal Children's Hospital

Stuart Berzins Author Microbiology and Immunology

Related Projects (7)

2007 PROGRAM GRANT - (IMMUNE REGULATION, EFFECTOR FUNCTION AND HUMAN IMMUNOTHERAPY)

The immune system plays an important role in protecting the host from viral and bacterial infections, and inhibits cancer onset and progress..

IMMUNE REGULATION EFFECTOR FUNCTION AND THERAPY

We seek to understand how white blood cells detect and destroy disease, and how molecules of the immune system punch holes in diseased cells..

IMMUNE REGULATION EFFECTOR FUNCTION AND THERAPY

We seek to understand how white blood cells detect and destroy disease, and how molecules of the immune system punch holes in diseased cells..

RESEARCH FELLOWSHIP

Professor Godfrey is an immunologist with a long standing history as a pioneer in the study of a specialised type of white blood cell, known..

2007 PROGRAM GRANT - (IMMUNE REGULATION, EFFECTOR FUNCTION AND HUMAN IMMUNOTHERAPY)

The immune system plays an important role in protecting the host from viral and bacterial infections, and inhibits cancer onset and progress..

The development of immune NKT cells in humans.

NKT cells are a type of white blood cell that help to control the function of the immune system. Many studies have reported an association b..

R. DOUGLAS WRIGHT BIOMEDICAL CAREER DEVELOPMENT AWARD - (DEVELOPMENT AND FUNCTION OF NKT CELL SUBSETS IN HUMANS)

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Grants

Awarded by NHMRC

Funding Acknowledgements

The authors acknowledge the kind donation of tissue for research purposes by donors and their families. This research was supported by an NHMRC Project Grant (no. 454363) and an NHMRC Program Grant (no. 454569). S. P. B. was supported by an NHMRC Career Development Fellowship (no. 454731) and by the Australian Government Collaborative Research Network (CRN). S. P. B. is currently supported as a Dorevitch Senior Research Fellow (at FECRI) and as a Robert H. T. Smith Fellow (Uni of Ballarat). D. I. G. is supported by an NHMRC Senior Principal Research Fellowship (no. 1020770).