Enhanced liver progenitor cell survival and differentiation in vivo by spheroid implantation in a vascularized tissue engineering chamber

KK Yap; AM Dingle; JA Palmer; RS Dhillon; Z Lokmic; AJ Penington; GC Yeoh; WA Morrison; GM Mitchell

Journal article

Enhanced liver progenitor cell survival and differentiation in vivo by spheroid implantation in a vascularized tissue engineering chamber

KK Yap, AM Dingle, JA Palmer, RS Dhillon, Z Lokmic, AJ Penington, GC Yeoh, WA Morrison, GM Mitchell

Biomaterials | Published : 2013

DOI: 10.1016/j.biomaterials.2013.02.011

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Abstract

Liver tissue engineering is hampered by poor implanted cell survival due to inadequate vascularization and cell-cell/cell-matrix interactions. Here, we use liver progenitor cell (LPC) spheroids to enhance cell-cell/cell-matrix interactions, with implantation into an angiogenic in vivo mouse chamber. Spheroids were generated in vitro in methylcellulose medium. Day 2 spheroids were optimal for implantation (22,407 +/-645 cells/spheroid), demonstrating maximal proliferation (Ki67 immunolabeling) and minimal apoptosis (caspase-3 immunolabelling). In vivo chambers established bilaterally on epigastric vessels of immunodeficient mice were implanted with equivalent numbers of LPCs as a cell suspens..

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University of Melbourne Researchers

Kiryu Yap Author

Anthony Penington Author

Related Projects (1)

Tissue engineering functional liver tissue

Many patients suffering from severe liver disease require a liver transplant, but due to a shortage of liver donors, many die prior to liver..

Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

This work was funded by the St Vincent's Hospital Melbourne Research Endowment Fund, the National Health and Medical Research Council of Australia (Project Grant 1023187), and the Victorian State Government's Department of Innovation, Industry and Regional Development's Operational Infrastructure Support Program.