Journal article
Inhibition of telomerase activity by human immunodeficiency virus (HIV) nucleos(t)ide reverse transcriptase inhibitors: A potential factor contributing to HIV-associated accelerated aging
E Leeansyah, PU Cameron, A Solomon, S Tennakoon, P Velayudham, M Gouillou, T Spelman, A Hearps, C Fairley, DV Smit, AB Pierce, J Armishaw, SM Crowe, DA Cooper, KK Koelsch, JP Liu, J Chuah, SR Lewin
Journal of Infectious Diseases | Published : 2013
Abstract
Background. Human immunodeficiency virus (HIV)-infected patients on combination active antiretroviral therapy (cART) are at increased risk of age-related complications. We hypothesized that nucleos(t)ide reverse transcriptase inhibitors (NRTI) may contribute to accelerated aging in HIV-infected individuals on cART via inhibition of telomerase activity.Methods. Telomerase activity and telomere length (TL) were measured by quantitative polymerase chain reaction in vitro in activated peripheral blood mononuclear cells (PBMCs) cultured with NRTI and ex vivo in PBMCs from uninfected patients exposed to NRTI and from HIV-infected patients on NRTI-containing cART.Results. Lamivudine, abacavir, zido..
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Funding Acknowledgements
This work was supported by the Alfred Foundation and the Gold Coast Sexual Health Centre. S. R. L. is a National Health and Medical Research Council Practitioner Fellow. S. M. C. is a National Health and Medical Research Council Principal Research Fellow. The authors gratefully acknowledge the contribution to this work of the Victorian Operational Infrastructure Support Program received by the Burnet Institute.