Journal article
The development and fate of follicular helper T cells defined by an IL-21 reporter mouse
K Lüthje, A Kallies, Y Shimohakamada, GT Belz, A Light, DM Tarlinton, SL Nutt
Nature Immunology | Published : 2012
DOI: 10.1038/ni.2261
Abstract
Germinal centers require CD4+ follicular helper T cells (T FH cells), whose hallmark is expression of the transcriptional repressor Bcl-6, the chemokine receptor CXCR5 and interleukin 21 (IL-21). To track the development and fate of TFH cells, we generated an IL-21 reporter mouse by introducing sequence encoding green fluorescent protein (GFP) into the Il21 locus; these mice had expression of IL-21-GFP in CD4 +CXCR5+PD-1+ TFH cells. IL-21-GFP+ TFH cells were multifunctional helper cells that coexpressed several cytokines, including interferon-γ (IFN-γ), IL-2 and IL-4. TFH cells proliferated and gave rise to transferrable memory cells with plasticity, which differentiated after recall into co..
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Funding Acknowledgements
We thank L. Corcoran (Walter and Eliza Hall Institute of Medical Research) for antibody to Bcl-6 and antibody to IRF4; and E. Cretney for preliminary experiments. Supported by the National Health and Medical Research Council of Australia (A. K., G. T. B., D. M. T. and S.L.N.), the German Academic Exchange Service (K. L.), the Sylvia and Charles Viertel Foundation (G. T. B.), the Howard Hughes Medical Institute (G. T. B.), Pfizer Australia Research (S.L.N.), the Australian Research Council (A. K. and S.L.N.), the Victorian State Government Operational Infrastructure Support and the National Health and Medical Research Council Independent Research Institutes Infrastructure Support Scheme of the Australian Government.