Ceramides Contained in LDL Are Elevated in Type 2 Diabetes and Promote Inflammation and Skeletal Muscle Insulin Resistance
James Boon, Andrew J Hoy, Romana Stark, Russell D Brown, Ruth C Meex, Darren C Henstridge, Simon Schenk, Peter J Meikle, Jeffrey F Horowitz, Bronwyn A Kingwell, Clinton R Bruce, Matthew J Watt
Diabetes | AMER DIABETES ASSOC | Published : 2013
Dysregulated lipid metabolism and inflammation are linked to the development of insulin resistance in obesity, and the intracellular accumulation of the sphingolipid ceramide has been implicated in these processes. Here, we explored the role of circulating ceramide on the pathogenesis of insulin resistance. Ceramide transported in LDL is elevated in the plasma of obese patients with type 2 diabetes and correlated with insulin resistance but not with the degree of obesity. Treating cultured myotubes with LDL containing ceramide promoted ceramide accrual in cells and was accompanied by reduced insulin-stimulated glucose uptake, Akt phosphorylation, and GLUT4 translocation compared with LDL def..View full abstract
Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
This study was supported in part by research grants and fellowships from the National Health and Medical Research Council of Australia (A.J.H., M.J.W., B.A.K., C.R.B., and P.J.M.), a Monash Fellowship (M.J.W.), and a National Heart Foundation Biomedical Postdoctoral Fellowship (D.C.H.).