Journal article

TLR4, IL10RA, and NOD2 mutation in paediatric Crohn's disease patients: an association with Mycobacterium avium subspecies paratuberculosis and TLR4 and IL10RA expression

Josef Wagner, Narelle A Skinner, Anthony G Catto-Smith, Donald JS Cameron, Wojtek P Michalski, Kumar Visvanathan, Carl D Kirkwood



Mycobacterium avium subspecies paratuberculosis (MAP) has been implicated in the pathogenesis of Crohn's disease (CD). The role of CD susceptibility genes in association with these microbes is not known. Sixty-two early onset paediatric CD patients and 46 controls with known MAP status were analysed for an association with 34 single nucleotide polymorphisms (SNPs) from 18 CD susceptibility genes. Functional studies on peripheral blood mononuclear cells (PBMCs) were conducted on 17 CD patients with known CD mutations to assess IL-6, IL-10, and TNF-α expression upon stimulation with MAP precipitated protein derivative (PPD) and lipopolysaccharide (LPS). In addition, surface expression of IL10R..

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Awarded by NHMRC CDA Fellowship

Funding Acknowledgements

We thank the children and their families for their participation in this study. This work was supported by research grants from the Murdoch Childrens Research Institute, The CASS Foundation, and The Lynne Quayle Charitable Trust, Equity Trustees LTD. Dr. C. Kirkwood is supported by an NHMRC CDA Fellowship (609347). This work was supported by the Victorian Government Operational Infrastructure Support Program.