Molecular chaperone heat shock protein 70 participates in the labile phase of the development of behavioural sensitization induced by a single morphine exposure in mice
Wang-Jun Qin, Yan-Ting Wang, Min Zhang, Rui-Ting Wen, Qing Liu, Yu-Ling Li, Feng Chen, Andrew J Lawrence, Jian-Hui Liang
INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY | OXFORD UNIV PRESS | Published : 2013
De-novo protein synthesis is required in the development of behavioural sensitization. A prior screening test from our laboratory has implicated heat shock protein 70 (Hsp70) as one of the proteins required in this behavioural plasticity. Thus, this study was designed to extend our understanding of the role of Hsp70 in the development of behavioural sensitization induced by a single morphine exposure in mice. First, by employing transcription inhibitor actinomycin D (AD) and protein synthesis inhibitor cycloheximide (CHX), we identified a protein synthesis-dependent labile phase (within 4 h after the first morphine injection) in the development of behavioural sensitization to a single morphi..View full abstract
Awarded by National Nature Science Foundation of China
Awarded by National Basic Research Program of China
Awarded by National Key Technology R&D Program of China
This study was supported by grants from National Nature Science Foundation of China (No. 30870894), National Basic Research Program of China (No. 2009CB522000) and National Key Technology R&D Program of China (No. 2011BAK04B08). Andrew J. Lawrence is a principal research fellow supported by the National Health & Medical Research Council (Australia).