Journal article
Differentiation of human adipose-derived stem cells into fat involves reactive oxygen species and forkhead box o1 mediated upregulation of antioxidant enzymes
M Higuchi, GJ Dusting, H Peshavariya, F Jiang, STF Hsiao, EC Chan, GS Liu
Stem Cells and Development | Published : 2013
Abstract
Both reactive oxygen species (ROS) and Forkhead box O (FOXO) family transcription factors are involved in the regulation of adipogenic differentiation of preadipocytes and stem cells. While FOXO has a pivotal role in maintaining cellular redox homeostasis, the interactions between ROS and FOXO during adipogenesis are not clear. Here we examined how ROS and FOXO regulate adipogenesis in human adipose-derived stem cells (hASC). The identity of isolated cells was confirmed by their surface marker expression pattern typical for human mesenchymal stem cells (positive for CD29, CD44, CD73, CD90, and CD105, negative for CD45 and CD31). Using a standard adipogenic cocktail consisting of insulin, dex..
View full abstractGrants
Awarded by National Health and Medical Research Council of Australia
Awarded by National Natural Science Foundation of China
Funding Acknowledgements
This work was supported by project grants from the National Health and Medical Research Council of Australia (NHMRC 09007G) (G. L.), the JO&JR Wicking Trust, and the National Natural Science Foundation of China (NSFC81070164) (F. J.). G. J. D. is supported by a principal research fellowship from NHMRC. Centre for Eye Research Australia and O'Brien Institute acknowledges the Victorian State Government's Department of Innovation, Industry and Regional Development's Operational Infrastructure Support Program.