The PI(3)P interactome from a colon cancer cell

B Catimel; E Kapp; MX Yin; M Gregory; LSM Wong; M Condron; N Church; N Kershaw; AB Holmes; AW Burgess

Journal article

The PI(3)P interactome from a colon cancer cell

B Catimel, E Kapp, MX Yin, M Gregory, LSM Wong, M Condron, N Church, N Kershaw, AB Holmes, AW Burgess

Journal of Proteomics | Published : 2013

DOI: 10.1016/j.jprot.2013.01.031

Abstract

A comprehensive analysis of the phosphoinositide interactome has been performed using an ω-amino analogue of phosphatidylinositol 3-phosphate (PI(3)P immobilised onto Affi-10 beads for use as an affinity absorbent for cytosolic, membrane and nuclear extracts from the LIM1215 colonic carcinoma cell line. Affinity/LC/MS/MS experiments allowed the identification of 681 proteins/protein complexes which interact with PI(3)P. Protein domain enrichment analysis identified proteins possessing PI(3)P (e.g., FYVE, PX, PH), PIP and PIP/phospholipid binding domains along with small GTPases, GTPase regulators, kinases and SH2/SH3 containing proteins. Functional and pathway enrichment analyses highlighted..

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University of Melbourne Researchers

Nadia Kershaw Author

Andrew Holmes Author

Antony Burgess Author

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Grants

Awarded by Australian Research Council

Funding Acknowledgements

This work was supported by the Australian Research Council, Discovery Project, grant DP1094497, the NHMRC Program grant 487922 and by funds from the Operational Infrastructure Support Program provided by the Victorian Government, Australia.