Journal article
Annexin Peptide Ac2-26 Suppresses TNFα-Induced Inflammatory Responses via Inhibition of Rac1-Dependent NADPH Oxidase in Human Endothelial Cells
HM Peshavariya, CJ Taylor, C Goh, GS Liu, F Jiang, EC Chan, GJ Dusting
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2013
Abstract
The anti-inflammatory peptide annexin-1 binds to formyl peptide receptors (FPR) but little is known about its mechanism of action in the vasculature. Here we investigate the effect of annexin peptide Ac2-26 on NADPH oxidase activity induced by tumour necrosis factor alpha (TNFα) in human endothelial cells. Superoxide release and intracellular reactive oxygen species (ROS) production from NADPH oxidase was measured with lucigenin-enhanced chemiluminescence and 2′,7′-dichlorodihydrofluorescein diacetate, respectively. Expression of NADPH oxidase subunits and intracellular cell adhesion molecule (ICAM-1) and vascular cell adhesion molecule (VCAM-1) were determined by real-time PCR and Western b..
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Funding Acknowledgements
This work was supported by Project Grants from the National Health and Medical Research Council (NHMRC) of Australia and Grants-in-Aid from the National Heart Foundation of Australia. GJD also receives a Principal Research Fellowship from NHMRC. The O'Brien Institute and Center for Eye Research Australia acknowledges the Victorian State Government's Department of Innovation, Industry and Regional Development's Operational Infrastructure Support Program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.