Exploring the utility of human DNA methylation arrays for profiling mouse genomic DNA
Nicholas C Wong, Jane Ng, Nathan E Hall, Sebastian Lunke, Marika Salmanidis, Gabriela Brumatti, Paul G Ekert, Jeffrey M Craig, Richard Saffery
GENOMICS | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2013
Illumina Infinium Human Methylation (HM) BeadChips are widely used for measuring genome-scale DNA methylation, particularly in relation to epigenome-wide association studies (EWAS) studies. The methylation profile of human samples can be assessed accurately and reproducibly using the HM27 BeadChip (27,578 CpG sites) or its successor, the HM450 BeadChip (482,421 CpG sites). To date no mouse equivalent has been developed, greatly hindering the application of this methodology to the wide range of valuable murine models of disease and development currently in existence. We found 1308 and 13,715 probes from HM27 and HM450 BeadChip respectively, uniquely matched the bisulfite converted reference m..View full abstract
Awarded by Victorian Life Sciences Computation Initiative (VLSCI) on the Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government, Australia
This research was supported by a Victorian Life Sciences Computation Initiative (VLSCI) grant number [VR0002] on the Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government, Australia.The Leukaemia Foundation of Australia supported this work through a Philip Desbrow Postdoctoral Fellowship (NW) and a Grant-in-Aid (NW, RS, and JC). NW and JC are currently supported by an NHMRC Project Grant and a Victorian Cancer Agency Grant. NW is also supported by the My Room and the Children's Cancer Centre Foundation. RS is supported by an NHMRC Senior Research Fellowship. MCRI is supported by the Victorian Government's Operational Infrastructure Support Program.