cAMP Response Element Binding Protein1 Is Essential for Activation of Steroyl Co-Enzyme A Desaturase 1 (Scd1) in Mouse Lung Type II Epithelial Cells

Abstract

Cyclic AMP Response Element-Binding Protein 1 (Creb1) is a transcription factor that mediates cyclic adenosine 3′, 5′-monophosphate (cAMP) signalling in many tissues. Creb1-/- mice die at birth due to respiratory failure and previous genome-wide microarray analysis of E17.5 Creb1-/- fetal mouse lung identified important Creb1-regulated gene targets during lung development. The lipogenic enzymes stearoyl-CoA desaturase 1 (Scd1) and fatty acid synthase (Fasn) showed highly reduced gene expression in Creb1-/- lungs. We therefore hypothesized that Creb1 plays a crucial role in the transcriptional regulation of genes involved in pulmonary lipid biosynthetic pathways during lung development. In th..