Journal article

Relaxin-3 innervation of the intergeniculate leaflet of the rat thalamus - neuronal tract-tracing and in vitro electrophysiological studies

Anna Blasiak, Tomasz Blasiak, Marian H Lewandowski, Mohammed Akhter Hossain, John D Wade, Andrew L Gundlach



Behavioural state is controlled by a range of neural systems that are sensitive to internal and external stimuli. The relaxin-3 and relaxin family peptide receptor 3 (RXFP3) system has emerged as a putative ascending arousal network with putative involvement in regulation of stress responses, neuroendocrine control, feeding and metabolism, circadian activity and cognition. Relaxin-3/γ-aminobutyric acid neuron populations have been identified in the nucleus incertus, pontine raphe nucleus, periaqueductal grey (PAG) and an area dorsal to the substantia nigra. Relaxin-3-positive fibres/terminals densely innervate arousal-related structures in the brainstem, hypothalamus and limbic forebrain, bu..

View full abstract


Awarded by Polish Ministry of Science and Higher Education

Awarded by National Health and Medical Research Council (NHMRC) of Australia

Funding Acknowledgements

This work was supported by: a research grant from the Polish Ministry of Science and Higher Education (N N303 569939 to T.B., A.B. and A.L.G.); a Commonwealth of Australia Endeavour Research Fellowship (A.B.); research grants from the National Health and Medical Research Council (NHMRC) of Australia (277609, 509246 and 1005988 to A.L.G.); a grant from the Florey Medical Research Foundation (A.L.G.); and the Victorian Government Operational Infrastructure Support Program. A.L.G. is an NHMRC (Australia) Senior Research Fellow. The authors would like to thank the Confocal Microscopy Laboratory, Institute of Zoology, Jagiellonian University for use of the LSM 510 META, Axiovert 200 M, ConfoCor 3 microscope (Carl Zeiss MicroImaging GmbH, Jena, Germany).