Journal article

In-vivo stimulation of macaque natural killer T cells with -galactosylceramide

CS Fernandez, S Jegaskanda, DI Godfrey, SJ Kent

CLINICAL AND EXPERIMENTAL IMMUNOLOGY | WILEY | Published : 2013

Abstract

Natural killer T cells are a potent mediator of anti-viral immunity in mice, but little is known about the effects of manipulating NKT cells in non-human primates. We evaluated the delivery of the NKT cell ligand, α-galactosylceramide (α-GalCer), in 27 macaques by studying the effects of different dosing (1-100 μg), and delivery modes [directly intravenously (i.v.) or pulsed onto blood or peripheral blood mononuclear cells]. We found that peripheral NKT cells were depleted transiently from the periphery following α-GalCer administration across all delivery modes, particularly in doses of ≥10 μg. Furthermore, NKT cell numbers frequently remained depressed at i.v. α-GalCer doses of >10 μg. Lev..

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Grants

Awarded by Australian National Health and Medical Research (NHMRC)


Awarded by NHMRC


Funding Acknowledgements

We thank Angela Chan, Thakshila Amarasena, Marcin Chiula, Sheilajen Alcantara, Kon Kyparissoudis and Wendy Winnall, and Drs Karen Laurie and Aeron Hurt of the World Health Organization (WHO) for expert assistance and advice. This research was supported by the Australian National Health and Medical Research (NHMRC) awards 510448, 629000 and 454569. D.I.G. is supported by an NHMRC senior principal research fellowship 454309; S.J.K. is supported by an NHMRC research fellowship 508937.