Journal article
Regulation of asymmetric cell division and polarity by Scribble is not required for humoral immunity
ED Hawkins, J Oliaro, A Kallies, GT Belz, A Filby, T Hogan, N Haynes, KM Ramsbottom, V Van Ham, T Kinwell, B Seddon, D Davies, D Tarlinton, AM Lew, PO Humbert, SM Russell
Nature Communications | Published : 2013
DOI: 10.1038/ncomms2796
Abstract
The production of protective antibody requires effective signalling of naive B cells following encounter with antigen, and the divergence of responding B lymphocytes into distinct lineages. Polarity proteins have recently been proposed as important mediators of both the initial B cell response, and potentially of asymmetric cell division. Here we show that, although polarity proteins of the Scribble complex, Scribble, Dlg1 and Lgl1, are expressed and polarized during early B cell activation, their deficiency has no effect on the in vivo outcome of immunization or challenge with influenza infection. Furthermore, we find a striking correlation in the differentiation outcome of daughters of sin..
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Awarded by National Institutes of Health
Funding Acknowledgements
We thank Valera Vasioukhin (Fred Hutchinson Cancer Research Centre) and Georgina Caruana (Monash University) for the Lgl and Dlg1-deficient mice, Daniel Day (Swinburne University) and Microsurfaces Pty Ltd for the cell paddocks. SW-Hel mice were provided by Robert Brink (Garvan Institute, Sydney, Australia). The work was funded by the Australian National Health and Medical Research Council (NHRMC, project grants and fellowships to EDH, JO, SMR, POH), the Human Frontiers Science Program, the Australian Research Council (ARC, fellowship to SMR), the Australian Cancer Research Foundation (ACRF, ACRF Cell Biology Program), NIH funding R01AI093870 (TH) and MRC UK programme funding U117573801 (BS). We also thank Olivia Cakebread, Michael Durrant, Josh Noske and Samantha Williams for animal husbandry.