Stiffness-mediated adhesion of cervical cancer cells to soft hydrogel films
James P Best, Sameen Javed, Joseph J Richardson, Kwun Lun Cho, Marloes MJ Kamphuis, Frank Caruso
SOFT MATTER | ROYAL SOC CHEMISTRY | Published : 2013
We report engineered hydrogel thin-films with varying degrees of covalent crosslinking, which demonstrate enhanced HeLa cell adhesion with decreasing film stiffness. This trend is contrary to previous findings for tumour cell adhesion on hydrogel substrates, and is attributed to the extremely soft nature of the films studied, allowing for a greater cell-film contact area and the development of adhesive focal contacts. Adhesion based on mechanical tuning of the film was decoupled from chemical effects through characterisation and analysis of film surface roughness, hydrophobicity and charge. © 2013 The Royal Society of Chemistry.
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Awarded by Australian Research Council
This work was supported by the Australian Research Council under the Federation Fellowship (FF0776078, F. C.), Australian Laureate Fellowship (FL120100030, F. C.) and Discovery Project (DP0877360, F. C.) schemes. The authors thank Dr Henk Dam for assistance with NMR spectroscopy measurements.