Gut microbial diversity is reduced and is associated with colonic inflammation in a piglet model of short bowel syndrome
Susan Lapthorne, Prue M Pereira-Fantini, Fiona Fouhy, Guineva Wilson, Sarah L Thomas, Nicole L Dellios, Michelle Scurr, Orla O'Sullivan, R Paul Ross, Catherine Stanton, Gerald F Fitzgerald, Paul D Cotter, Julie E Bines
Gut Microbes | TAYLOR & FRANCIS INC | Published : 2013
BACKGROUND AND OBJECTIVES: Following small bowel resection (SBR), the luminal environment is altered, which contributes to clinical manifestations of short bowel syndrome (SBS) including malabsorption, mucosal inflammation and bacterial overgrowth. However, the impact of SBR on the colon has not been well-defined. The aims of this study were to characterize the colonic microbiota following SBR and to assess the impact of SBR on mucosal inflammation in the colon. RESULTS: Analysis of the colonic microbiota demonstrated that there was a significant level of dysbiosis both two and six weeks post-SBR, particularly in the phylum Firmicutes, coupled with a decrease in overall bacterial diversity i..View full abstract
This work was supported by the Victorian Government's Operational Infrastructure Support Program. F.F. is in receipt of an Irish Research Council for Science, Engineering and Technology EMBARK scholarship and is a Teagasc Walsh fellow. Research in the Ross, Fitzgerald, Stanton and Cotter laboratories is funded by Science Foundation Ireland (in the form of a research center grant to the Alimentary Pharmabiotic Centre and a PI award to P.C.). The authors wish to thank Magdy Sourial, Shane Osterfield and Dr Andrew French for expert technical assistance with the animals and Dr Jean-Pierre Scheerlinck and The University of Melbourne Centre for Animal Biotechnology for the use of their facilities. The authors would also like to thank Dr Eva Rosberg-Cody for high-throughput DNA sequencing services.