Electrochemical investigation of the interaction between lysozyme-shelled microbubbles and vitamin C

Abstract

We report loading of vitamin C (ascorbic acid) on to lysozyme-shelled microbubbles. The interaction between lysozyme-shelled microbubbles and vitamin C was studied by use of cyclic and differential pulse voltammetry, zeta potential measurements, and scanning electron microscopy. The effect of microbubbles on electrochemical measurement of ascorbic acid was evaluated. The linear range for ascorbic acid obtained for differential pulse measurement in the presence of 1 mg mL-1 microbubbles was 1-50 μmol L -1 (y = 0.067x + 0.130, r 2 = 0.995), with a detection limit of 0.5 μmol L-1. The experimental conditions, i.e., pH and ionic strength, were optimized to improve the interaction between ascorbi..