Wnt Signaling Orchestration with a Small Molecule DYRK Inhibitor Provides Long-Term Xeno-Free Human Pluripotent Cell Expansion
Kouichi Hasegawa, Shin-ya Yasuda, Jia-Ling Teo, Nguyen Cu, Michael McMillan, Chih-Lin Hsieh, Hirofumi Suemori, Norio Nakatsuji, Masashi Yamamoto, Tomoyuki Miyabayashi, Carolyn Lutzko, Martin F Pera, Michael Kahn
Stem Cells Translational Medicine | WILEY | Published : 2012
An optimal culture system for human pluripotent stem cells should be fully defined and free of animal components. To date, most xeno-free culture systems require human feeder cells and/or highly complicated culture media that contain activators of the fibroblast growth factor (FGF) and transforming growth factor-β (TGFβ) signaling pathways, and none provide for replacement of FGF/TGFβ ligands with chemical compounds. The Wnt/β-catenin signaling pathway plays an important role in mouse embryonic stem cells in leukemia inhibitory factor-independent culture; however, the role of Wnt/β-catenin signaling in human pluripotent stem cell is still poorly understood and controversial because of the du..View full abstract
Awarded by New Energy and Industrial Technology Development Organization (NEDO) Japan
Awarded by California Institute for Regenerative Medicine
We thank the Stem Cell Core facilities at Children's Hospital Los Angeles and at the University of Southern California for providing hESCs. Work at Kyoto University was supported by New Energy and Industrial Technology Development Organization (NEDO) Japan, Grant #P10027 Fundamental Technology Development for Promoting Industrial Application of Human Stem Cells. Work at the University of Southern California was supported by California Institute for Regenerative Medicine, New Cell Lines Grant #RL1-00667-1, New Technology for the Derivation of Human Pluripotent Stem Cell Lines for Clinical Use.