Journal article

Global analysis of outer membrane proteins from Leptospira interrogans serovar Lai

PA Cullen, SJ Cordwell, DM Bulach, DA Haake, B Adler

INFECTION AND IMMUNITY | AMER SOC MICROBIOLOGY | Published : 2002

Abstract

Recombinant leptospiral outer membrane proteins (OMPs) can elicit immunity to leptospirosis in a hamster infection model. Previously characterized OMPs appear highly conserved, and thus their potential to stimulate heterologous immunity is of critical importance. In this study we undertook a global analysis of leptospiral OMPs, which were obtained by Triton X-114 extraction and phase partitioning. Outer membrane fractions were isolated from Leptospira interrogans serovar Lai grown at 20, 30, and 37 degrees C with or without 10% fetal calf serum and, finally, in iron-depleted medium. The OMPs were separated by two-dimensional gel electrophoresis. Gel patterns from each of the five conditions ..

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University of Melbourne Researchers