Journal article
Signal regulatory protein molecules are differentially expressed, by CD8- dendritic cells
MH Lahoud, AI Proietto, KH Gartlan, S Kitsoulis, J Curtis, J Wettenhall, M Sofi, C Daunt, M O'Keeffe, I Caminschi, K Satterley, A Rizzitelli, P Schnorrer, A Hinohara, Y Yamaguchi, L Wu, G Smyth, E Handman, K Shortman, MD Wright
Journal of Immunology | AMER ASSOC IMMUNOLOGISTS | Published : 2006
Abstract
A normalized subtracted gene expression library was generated from freshly isolated mouse dendritic cells (DC) of all subtypes, then used to construct cDNA microarrays. The gene expression profiles of the three splenic conventional DC (cDC) subsets were compared by microarray hybridization and two genes encoding signal regulatory protein β (Sirpβ1 and Sirpβ4) molecules were identified as differentially expressed in CD8- cDC. Genomic sequence analysis revealed a third Sirpβ member localized in the same gene cluster. These Sirpβ genes encode cell surface molecules containing extracellular Ig domains and short intracytoplasmic domains that have a charged amino acid in the transmembrane region w..
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