Journal article
Acute Rho-kinase inhibition improves coronary dysfunction in vivo, in the early diabetic microcirculation.
JT Pearson, MJ Jenkins, AJ Edgley, T Sonobe, M Joshi, MT Waddingham, Y Fujii, DO Schwenke, H Tsuchimochi, M Yoshimoto, K Umetani, DJ Kelly, M Shirai
Cardiovascular Diabetology | Published : 2013
Open access
Abstract
Activation of RhoA/Rho-kinase (ROCK) is increasingly implicated in acute vasospasm and chronic vasoconstriction in major organ systems. Therefore we aimed to ascertain whether an increase in ROCK activity plays a role in the deterioration of coronary vascular function in early stage diabetes. Synchrotron radiation microangiography was used to determine in vivo coronary responses in diabetic (3 weeks post streptozotocin 65 mg/kg ip) and vehicle treated male Sprague-Dawley rats (n = 8 and 6). Changes in vessel number and calibre during vasodilator stimulation before and after blockade of nitric oxide synthase and cyclooxygenase were compared between rats. Acute responses to ROCK inhibitor, fas..
View full abstractGrants
Awarded by Australian Government
Funding Acknowledgements
Experiments were conducted at SPring-8, Japan Synchrotron Radiation Research Institute, Hyogo, Japan (Proposals 2009A1467, 2010A1260, 2010B1372) with approval from the Animal Experiment Review Committee. DJK was supported by a National Health and Medical Research Council Senior Research Fellowship. The authors acknowledge support from the International Synchrotron Access Programme (IA104) managed by the Australian Synchrotron and funded by the Australian Government, Intramural Research Funds (22-2-3, 22-3-2) of the National Cerebral and Cardiovascular Center, Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (16659210, 20590242, 23249038, 23650213). The authors are grateful to Kath MacLeod for comments on an earlier draft of this manuscript.