Short-term single treatment of chemotherapy results in the enrichment of ovarian cancer stem cell-like cells leading to an increased tumor burden
Khalid Abubaker, Ardian Latifi, Rod Luwor, Simon Nazaretian, Hongjian Zhu, Michael A Quinn, Erik W Thompson, Jock K Findlay, Nuzhat Ahmed
MOLECULAR CANCER | BMC | Published : 2013
Over 80% of women diagnosed with advanced-stage ovarian cancer die as a result of disease recurrence due to failure of chemotherapy treatment. In this study, using two distinct ovarian cancer cell lines (epithelial OVCA 433 and mesenchymal HEY) we demonstrate enrichment in a population of cells with high expression of CSC markers at the protein and mRNA levels in response to cisplatin, paclitaxel and the combination of both. We also demonstrate a significant enhancement in the sphere forming abilities of ovarian cancer cells in response to chemotherapy drugs. The results of these in vitro findings are supported by in vivo mouse xenograft models in which intraperitoneal transplantation of cis..View full abstract
Awarded by National Health and Medical Research Council of Australia
The authors wish to thank Royal Women's Hospital Foundation, Women's Cancer Foundation, National Health and Medical Research Council of Australia (JKF, RegKey#441101) and the Victorian Government's Operational Infrastructure Support Program and National Breast Cancer Foundation (EWT) for supporting this work. AL and KA are the recipients of Royal Women's Hospital Scholarship and Australian Postgraduate Award.