Journal article
Serial study of lymph node cell subsets using fine needle aspiration in pigtail macaques
Y Xu, C Fernandez, S Alcantara, M Bailey, R De Rose, AD Kelleher, J Zaunders, SJ Kent
Journal of Immunological Methods | Published : 2013
Abstract
Lymphoid tissues are of intense interest for studies of the pathogenesis of human immunodeficiency virus (HIV) in humans and simian immunodeficiency virus (SIV) in macaques but are relatively difficult to sample non-invasively. Fine needle aspiration (FNA) cytology, conventionally a diagnostic procedure for lymphadenopathy, can be used for longitudinal study of tissue cell subsets during HIV/SIV infection. In this study, we serially sampled lymph node (LN) FNA from pigtail macaques and studied cell subsets in the aspect of absolute count, frequency, and functionality by flow cytometry. The median recovered lymphocyte count from FNA samples was 2.01×105 (3.0×103 to 2.25×106, n=38) and median ..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
We acknowledge Dr. Kersten Koelsch for his input into the design of the study. This work was supported by the Australian National Health and Medical Research Council program grant 510488. The Kirby Institute is funded by the Australian Government Department of Health and Ageing and is affiliated with the Faculty of Medicine, The University of New South Wales.