Expression of MUC2, MUC5AC, MUC5B, and MUC6 mucins in colorectal cancers and their association with the CpG island methylator phenotype
Michael D Walsh, Mark Clendenning, Elizabeth Williamson, Sally-Ann Pearson, Rhiannon J Walters, Belinda Nagler, David Packenas, Aung K Win, John L Hopper, Mark A Jenkins, Andrew M Haydon, Christophe Rosty, Dallas R English, Graham G Giles, Michael A McGuckin, Joanne P Young, Daniel D Buchanan
MODERN PATHOLOGY | NATURE PUBLISHING GROUP | Published : 2013
Mucinous differentiation is associated with both CpG island methylator phenotype and microsatellite instability in colorectal cancer. The mucinous phenotype derives from abundant expression of the colonic goblet cell mucin, MUC2, and de novo expression of gastric foveolar mucin, MUC5AC. We, therefore, investigated the protein expression levels of MUC2 and MUC5AC, as well as MUC5B and MUC6, in molecular subtypes of colorectal cancer. Seven-hundred and twenty-two incident colorectal carcinomas occurring in 702 participants of the Melbourne Collaborative Cohort Study were characterized for methylator status, MLH1 methylation, somatic BRAF and KRAS mutations, microsatellite-instability status, M..View full abstract
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Awarded by NHMRC
We thank Charmaine Smith, Lisa Oates, and Sonia Terre'Blanche from the Cancer Council Victoria for their tireless assistance with tissue block acquisition, Diane McKeone and Erika Pavluk for technical and database assistance, the late Professor Jeremy jass for performing some of the histopathology reviews, and Dr Karine Rousseau (University of Manchester, UK) for her gift of the MUC5B monoclonal antibody used in the study. The authors are also indebted to Heather Matthews for providing graphics support. We would like to thank the various clinical laboratories that very kindly made access to tissue blocks possible. This study was supported by a grant from the NHMRC. Christophe Rosty is the Jass Pathology Fellow, John L Hopper is an NMHRC Australia Fellow, and Mark A Jenkins and Michael A McGu ckin are supported by NHMRC Senior Research Fellowships. MCCS recruitment was funded by Vic Health, and ongoing support has been provided from recurrent funding by Cancer Council Victoria. This work was funded in part by a NHMRC Program Grant (APP209057).