Journal article

The Dot/Icm Effector SdhA Is Necessary for Virulence of Legionella pneumophila in Galleria mellonella and A/J Mice

Clare R Harding, Charlotte A Stoneham, Ralf Schuelein, Hayley Newton, Clare V Oates, Elizabeth L Hartland, Gunnar N Schroeder, Gad Frankel



Legionella pneumophila is an intracellular bacterium that resides within amoebae and macrophages in a specialized compartment termed the Legionella-containing vacuole (LCV). As well as providing an intracellular niche for replication, the LCV helps to prevent the release of bacterial components into the cytoplasm. Recognition of these components as danger signals by the host activates immune responses leading to clearance of the bacterium. Here, we examined the role of two important virulence factors of L. pneumophila, the potent danger signal flagellin and the translocated Dot/Icm type IVB secretion system effector SdhA, which is crucial to maintain LCV integrity, in the Galleria mellonella..

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Awarded by Medical Research Council

Funding Acknowledgements

Transmission electron microscopy was performed at the Henry Wellcome Imaging Centre, Division of Infectious Diseases, St. Mary's Hospital Campus, Imperial College London. This work was supported by grants from the Wellcome Trust, the Medical Research Council, the Australian National Health and Medical Research Council, and the Australian Research Council.