Proteomic Analysis of Oesophagostomum dentatum (Nematoda) during Larval Transition, and the Effects of Hydrolase Inhibitors on Development
Martina Ondrovics, Katja Silbermayr, Makedonka Mitreva, Neil D Young, Ebrahim Razzazi-Fazeli, Robin B Gasser, Anja Joachim
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2013
In this study, in vitro drug testing was combined with proteomic and bioinformatic analyses to identify and characterize proteins involved in larval development of Oesophagostomum dentatum, an economically important parasitic nematode. Four hydrolase inhibitors ο-phenanthroline, sodium fluoride, iodoacetamide and 1,2-epoxy-3-(pnitrophenoxy)-propane (EPNP) significantly inhibited (≥90%) larval development. Comparison of the proteomic profiles of the development-inhibited larvae with those of uninhibited control larvae using two-dimensional gel electrophoresis, and subsequent MALDI-TOF mass spectrometric analysis identified a down-regulation of 12 proteins inferred to be involved in various la..View full abstract
Awarded by National Institutes of Health
MO is recipient of a DOC-fFORTE-fellowship of the Austrian Academy of Sciences. Funding support to RBG from the Australian Research Council (ARC), the National Health and Medical Research Council (NHMRC) and the Alexander von Humboldt Foundation is gratefully acknowledged. Work at Washington University is supported by National Institutes of Health grant AI081803 to MM. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.