Journal article
Direct in vivo imaging of essential bioinorganics in Caenorhabditis elegans
SA James, MD De Jonge, DL Howard, AI Bush, D Paterson, G McColl
Metallomics | ROYAL SOC CHEMISTRY | Published : 2013
DOI: 10.1039/c3mt00010a
Abstract
Metals are essential in biochemistry, with highly regulated localisation and concentration. To continue elucidating the impact of inorganic physiology on cellular process in health and disease requires development of suitable eukaryotic models and experimental approaches. The multicellular organism Caenorhabditis elegans has been widely adopted as a model system and proved to be highly valuable in biomedical research, and here we record the distribution of physiologically important 4th row elements throughout anesthetised, hydrated adult C. elegans without the need for chemical fixation or mechanical sectioning. Probing the partitioning of elements between intact anatomical structures at sub..
View full abstractRelated Projects (2)
Grants
Awarded by NIH Office of Research Infrastructure Programs
Funding Acknowledgements
We thank Nicole Jenkins and Kathryn Spiers for helpful comments and Dominic Hare for assistance forming schematics. This work was supported by funds from the Australian Research Council, the National Health and Medical Research Council of Australia, and Operational Infrastructure Support Victorian State Government, the CSIRO OCE Postdoctoral Fellowship Program and the Australian Synchrotron. This research was undertaken on the XFM beamline at the Australian Synchrotron (AS091/XFM1058), Victoria, Australia. C. elegans strains used in this study were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440).