Journal article

Mapping cation entry in photoreceptors and inner retinal neurons during early degeneration in the P23H-3 rat retina

Yuan Zhu, Stuti Mistra, Lisa Nivison-Smith, Monica L Acosta, Erica L Fletcher, Michael Kalloniatis



The proline-23-histidine line 3 (P23H-3) transgenic rat carries a human opsin gene mutation leading to progressive photoreceptor loss characteristic of human autosomal dominant retinitis pigmentosa. The aim of the present study was to evaluate neurochemical modifications in the P23H-3 retina as a function of development and degeneration. Specifically, we investigated the ion channel permeability of photoreceptors by tracking an organic cation, agmatine (1-amino-4-guanidobutane, AGB), which permeates through nonspecific cation channels. We also investigated the activity of ionotropic glutamate receptors in distinct populations of bipolar, amacrine, and ganglion cells using AGB tracking in com..

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University of Melbourne Researchers


Awarded by National Health and Medical Research Council, Australia

Awarded by Health Research Council of New Zealand, Retina Australia

Funding Acknowledgements

This work was supported in part by research grants from the National Health and Medical Research Council, Australia (1009342 and 1021042), the Health Research Council of New Zealand (05/247), Retina Australia and the Robert G. Leitl Trust (Professorship held by M.K.). We would like to thank Dr. Robert Marc, University of Utah, for providing the agmatine antibodies. The rats were produced by Xenogen Biosciences (formerly Chrysalis DNX Transgenic Sciences, Princeton, NJ) and developed and supplied with the support of the National Eye Institute by Professor Matthew LaVail (Beckman Vision Center, School of Medicine, University of California, San Francisco, CA). We thank Professor Jonathan Stone and Dr. Krisztina Valter-Kocsi for providing the P23H-3 breeders.