Journal article
Concordant mast cell and basophil production by individual hematopoietic blast colony-forming cells
D Metcalf, AP Ng, TM Baldwin, L Di Rago, S Mifsud
Proceedings of the National Academy of Sciences of the United States of America | Published : 2013
Abstract
Previous studies have shown that mouse bone marrow cells can produce mast cells when stimulated in vitro by stem cell factor (SCF) and interleukin-3 (IL-3). Experiments to define the marrow cells able to generate mast cells showed that the most active subpopulations were the Kit+Sca1 -progenitor cell fraction and the more ancestral Kit+ Sca1+ blast colony-forming cell fraction. In clonal cultures, up to 64% of blast colony-forming cells were able to generate mast cells when stimulated by SCF and IL-3, and, of these, the most active were those in the CD34-Flt3R-long-term repopulating cell fraction. Basophils, identified by the monoclonal antibody mMCP-8 to mouse mast cell serine protease-8, w..
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Awarded by National Health and the Medical Research Council, Canberra
Awarded by National Health and Medical Research Council (Australia)
Funding Acknowledgements
This work was supported by the Carden Fellowship Fund of the Cancer Council, Victoria; the National Health and the Medical Research Council, Canberra (Programs 461219 and 1016647); the National Health and Medical Research Council (Australia) Independent Research Institutes Infrastructure Support Scheme Grant 361646; and a Victorian State Government Operational Infrastructure Support grant. A.P.N. was a recipient of an Australian Postgraduate Award.