Journal article
Expression of the transmembrane lysosomal protein SCARB2/Limp-2 in renin secretory granules controls renin release
D Lee, MJ Desmond, SA Fraser, M Katerelos, K Gleich, SF Berkovic, DA Power
Nephron Experimental Nephrology | KARGER | Published : 2013
DOI: 10.1159/000350737
Abstract
Background/Aims: Renin processing and storage is believed to occur in lysosome-like structures in the afferent arteriole. SCARB2/Limp-2 is a transmembrane lysosomal protein responsible for the intracellular trafficking of β-glucocerebrosidase. This study aimed to confirm the expression of SCARB2/Limp-2 in renin secretory granules, and explore its role in renin processing and secretion. Methods: Co-localisation studies of (pro)renin with lysosomal membrane proteins, SCARB2/Limp-2, LAMP-1 and LAMP-2, were performed in mouse and human kidney sections. Intrarenal expression and secretion of (pro)renin in wild-type (WT) and Limp-2-/- mice were compared with and without stimulation. Results: SCARB..
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Funding Acknowledgements
D.L. has been supported by a Postgraduate Scholarship from the National Health and Medical Research Council, Australia, and Jacquot Bequest, Royal Australiasian College of Physicians. This work was supported by a National Health and Medical Research Council Project Grant to D. A. P. and a Program Grant to S.F.B.