Thrombopoietin signaling is required for in vivo expansion of IL-11-responsive hematopoietic progenitor cells in the steady state

Abstract

Objective - mpl-/- mice have a profound defect in platelets and megakaryocytes and a defect in hematopoietic progenitor cells and stem cells. However, no specific subset of the progenitor/stem cell compartment has been shown to be particularly affected by this deficiency in mpl-/- mice. In this article, we identified a specific subset of bone marrow progenitor/stem cells that was altered in mpl-/- mice. Materials and Methods - In vitro and in vivo hematopoietic assays were utilized to examine the response to interleukin-11 in mice lacking the receptor for thrombopoietin (TPO) (mpl-/- mice). Results - The interleukin (IL)-11-responsive subset of progenitor cells was not detected in clonal cul..