Journal article
High-resolution melting genotyping of enterococcus faecium based on multilocus sequence typing derived single nucleotide polymorphisms
SYC Tong, S Xie, LJ Richardson, SA Ballard, F Dakh, EA Grabsch, ML Grayson, BP Howden, PDR Johnson, PM Giffard
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2011
Abstract
We have developed a single nucleotide polymorphism (SNP) nucleated high-resolution melting (HRM) technique to genotype Enterococcus faecium. Eight SNPs were derived from the E. faecium multilocus sequence typing (MLST) database and amplified fragments containing these SNPs were interrogated by HRM. We tested the HRM genotyping scheme on 85 E. faecium bloodstream isolates and compared the results with MLST, pulsed-field gel electrophoresis (PFGE) and an allele specific real-time PCR (AS kinetic PCR) SNP typing method. In silico analysis based on predicted HRM curves according to the G+C content of each fragment for all 567 sequence types (STs) in the MLST database together with empiric data f..
View full abstractGrants
Awarded by Australian National Health and Medical Research Council
Funding Acknowledgements
This work was supported by an Australian National Health and Medical Research Council Postdoctoral Training Fellowship (508829) to ST and by the Austin Hospital Department of Pathology to SX. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.