Journal article
Identification and prioritization of merozoite antigens as targets of protective human immunity to Plasmodium falciparum malaria for vaccine and biomarker development
JS Richards, TU Arumugam, L Reiling, J Healer, AN Hodder, FJI Fowkes, N Cross, C Langer, S Takeo, AD Uboldi, JK Thompson, PR Gilson, RL Coppel, PM Siba, CL King, M Torii, CE Chitnis, DL Narum, I Mueller, BS Crabb Show all
Journal of Immunology | AMER ASSOC IMMUNOLOGISTS | Published : 2013
Abstract
The development of effective malaria vaccines and immune biomarkers of malaria is a high priority for malaria control and elimination. Ags expressed by merozoites of Plasmodium falciparum are likely to be important targets of human immunity and are promising vaccine candidates, but very few Ags have been studied. We developed an approach to assess Ab responses to a comprehensive repertoire of merozoite proteins and investigate whether they are targets of protective Abs. We expressed 91 recombinant proteins, located on the merozoite surface or within invasion organelles, and screened them for quality and reactivity to human Abs. Subsequently, Abs to 46 proteins were studied in a longitudinal ..
View full abstractGrants
Awarded by Japan Society for the Promotion of Science
Funding Acknowledgements
This work was supported by the National Health and Medical Research Council of Australia (a project grant and program grant, postgraduate research fellowship to J.S.R., a Training Award to F.J.I.F., and an Infrastructure for Research Institutes Support Scheme Grant); the Bill and Melinda Gates Foundation; the Australian Research Council (a Future Fellowship to J.G.B.); the Australia-India Strategic Research Fund of the Department of Innovation Industry Science and Research, Australia and Department of Biotechnology, India; Ministry of Education, Culture, Sports, Science and Technology Grants-in-Aid for Scientific Research (KAKENHI) (23117008); Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (KAKENHI) (23406007); the Ministry of Health, Labour, and Welfare, Japan (Grant H21-Chikyukibo-ippan-005); the Victorian State Government Operational Infrastructure Support; and the National Institutes of Health (to D.L.N.).