Journal article
HLA peptide length preferences control CD8 T cell responses
MJ Rist, A Theodossis, NP Croft, MA Neller, A Welland, Z Chen, LC Sullivan, JM Burrows, JJ Miles, RM Brennan, S Gras, R Khanna, AG Brooks, J McCluskey, AW Purcell, J Rossjohn, SR Burrows
Journal of Immunology | Published : 2013
Abstract
Class I HLAs generally present peptides of 8-10 aa in length, although it is unclear whether peptide length preferences are affected by HLA polymorphism. In this study, we investigated the CD8+ T cell response to the BZLF1 Ag of EBV, which includes overlapping sequences of different size that nevertheless conform to the binding motif of the large and abundant HLA-B*44 supertype. Whereas HLA-B*18:01+ individuals responded strongly and exclusively to the octamer peptide 173SELEIKRY180, HLA-B*44:03+ individuals responded to the atypically large dodecamer peptide 169EECDSELEIKRY180, which encompasses the octamer peptide. Moreover, the octamer peptide bound more stably to HLA-B*18:01 than did the..
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Funding Acknowledgements
This work was supported by the National Health and Medical Research Council (NHMRC) of Australia and the Australian Research Council (ARC). J.J.M. is supported by an NHMRC Career Development Fellowship, S.R.B. by an NHMRC Principal Research Fellowship, A.W.P. by an NHMRC Senior Research Fellowship, S.G. by an ARC Future Fellowship, and J.R. by an NHMRC Australia Fellowship.