Journal article
A comprehensive survey of single nucleotide polymorphisms (SNPs) across Mycobacterium bovis strains and M. bovis BCG vaccine strains refines the genealogy and defines a minimal set of SNPs that separate virulent M. bovis strains and M. bovis BCG strains
MCG Pelayo, S Uplekar, A Keniry, PM Lopez, T Garnier, JN Garcia, L Boschiroli, X Zhou, J Parkhill, N Smith, RG Hewinson, ST Cole, SV Gordon
Infection and Immunity | Published : 2009
DOI: 10.1128/IAI.01099-08
Abstract
To further unravel the mechanisms responsible for attenuation of the tuberculosis vaccine Mycobacterium bovis BCG, comparative genomics was used to identify single nucleotide polymorphisms (SNPs) that differed between sequenced strains of Mycobacterium bovis and M. bovis BCG. SNPs were assayed in M. bovis isolates from France and the United Kingdom and from different BCG vaccines in order to identify those that arose during the attenuation process which gave rise to BCG. Informative data sets were obtained for 658 SNPs from 21 virulent M. bovis strains and 13 BCG strains; these SNPs showed phylogenetic clustering that was consistent with the geographical origin of the strains and previous sc..
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Awarded by Department of Environment, Food and Rural Affairs
Awarded by BBSRC
Funding Acknowledgements
[ "This work was funded by the Department of Environment, Food and Rural Affairs (project SE3224); the Fondation Raoul Follereau; the Wellcome Trust; and SystemsX.ch. Xiangmei Zhou was funded by BBSRC grant CPA1497, \"Genomic and Post-genomics of Salmonella and Mycobacterium as paradigms of intracellular pathogens,\" awarded to Paul Barrow, University of Nottingham, United Kingdom.", "We also thank one anonymous referee for helpful comments." ]