Journal article

Selective Depletion of Cross-Presenting Dendritic Cells Enhances Islet Allograft Survival

Robyn M Sutherland, Yifan Zhan, Emma M Carrington, Sarah L Londrigan, Andrew M Lew

Cell Transplant. | COGNIZANT COMMUNICATION CORP | Published : 2011

Abstract

MHC class I presentation of peptides derived from exogenous antigens (not synthesized within the antigen-presenting cell) is called cross-presentation and is mediated by selective subsets of dendritic cells (DC). A proportion of both donor and host DC may cross-present. Although there has been many studies that have investigated the role of donor versus host DC (i.e., direct vs. indirect pathway), what role cross-presenting DC play in allograft rejection has not been determined. We recently identified an agent, cytochrome c (cytc), that selectively depletes cross-presenting DC in vivo. By administering cytc we were able to study the impact of cross-presenting DC on rejection of islets grafte..

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Grants

Awarded by NHMRC


Funding Acknowledgements

This work was supported by National Health and Medical Research Council of Australia (NH&MRC), Diabetes Australia, Juvenile Diabetes Research Foundation, NH&MRC Independent Research Institutes Infrastructure Support Scheme grant #361646, and Victorian State Government Operational Infrastructure Support grant. We are grateful to Nicole Ashman for technical assistance with mice, and to Steven Mihajlovic for help with histology.