Journal article
The Ecdysone receptor constrains wingless expression to pattern cell cycle across the Drosophila wing margin in a cyclin B-dependent manner
NC Mitchell, JI Lin, O Zaytseva, N Cranna, A Lee, LM Quinn
BMC Developmental Biology | BMC | Published : 2013
Abstract
Background: Ecdysone triggers transcriptional changes via the ecdysone receptor (EcR) to coordinate developmental programs of apoptosis, cell cycle and differentiation. Data suggests EcR affects cell cycle gene expression indirectly and here we identify Wingless as an intermediary factor linking EcR to cell cycle. Results: We demonstrate EcR patterns cell cycle across the presumptive Drosophila wing margin by constraining wg transcription to modulate CycB expression, but not the previously identified Wg-targets dMyc or Stg. Furthermore co-knockdown of Wg restores CycB patterning in EcR knockdown clones. Wg is not a direct target of EcR, rather we demonstrate that repression of Wg by EcR is l..
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Awarded by Cancer Council Victoria
Funding Acknowledgements
This work was supported by the Australian National Health and Medical Research Council (NHMRC) and Cancer Council Victoria (CCV). LQ, NM and JL are supported by NHMRC project grants. NC is supported by an Australian Postgraduate Award (APA). AL is supported by a Victorian Government International Scholarship. We thank the following researchers for reagents; Laura Johnston (UAS-myc and Act < CD2 < GAL4 UAS-GFP), Bruce Edgar (UAS-E2F1/UAS-DP), Robert Duronio (PCNA-GFP), Daniella Grifoni and Paola Bellosta (dMyc antibody), David Glover (Cyclin B antibody). We thank the Vienna Drosophila Resource Centre (VDRC), National Institute of Genetics, Japan (NIG), the Bloomington Stock Center, Sveged and The Carnegie Fly Trap collection for Drosophila strains and the Developmental Hybridoma Bank (DSHB) for antibodies.