Journal article

An Overexpression Screen of Toxoplasma gondii Rab-GTPases Reveals Distinct Transport Routes to the Micronemes

Katrin Kremer, Dirk Kamin, Eva Rittweger, Jonathan Wilkes, Halley Flammer, Sabine Mahler, Joanne Heng, Christopher J Tonkin, Gordon Langsley, Stefan W Hell, Vernon B Carruthers, David JP Ferguson, Markus Meissner

PLOS PATHOGENS | PUBLIC LIBRARY SCIENCE | Published : 2013

Abstract

The basic organisation of the endomembrane system is conserved in all eukaryotes and comparative genome analyses provides compelling evidence that the endomembrane system of the last common eukaryotic ancestor (LCEA) is complex with many genes required for regulated traffic being present. Although apicomplexan parasites, causative agents of severe human and animal diseases, appear to have only a basic set of trafficking factors such as Rab-GTPases, they evolved unique secretory organelles (micronemes, rhoptries and dense granules) that are sequentially secreted during invasion of the host cell. In order to define the secretory pathway of apicomplexans, we performed an overexpression screen o..

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Grants

Awarded by BioFuture-Programm of the German ministry of science and education (BMBF)


Awarded by Wellcome Trust Senior Fellowship


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

This work was funded by the BioFuture-Programm (Grant number: 0311897) of the German ministry of science and education (BMBF), by a Wellcome Trust Senior Fellowship for MM (Grant number: 087582/Z/08/Z), MALSIG and EviMalAr (European FP7). DJPF was supported by a Wellcome Trust equipment grant. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.